A New Fluorescence Based Method for the Characterization of In Vitro Aerosol Exposure Systems


Authored by  S Steiner, S Majeed, M Nordlund, S Frentzel, C Mathis, J Hoeng

Presented at IIVS Exposure-Dosimetry workshop     

Abstract

The Institute for In Vitro Sciences (IIVS) brought together stakeholders from industry, government and academia to discuss considerations for exposure and dosimetry for non-animal testing during its 2.5-day workshop, “In Vitro Exposure Systems and Dosimetry Assessment Tools for Inhaled Tobacco Products”, held April 4-6, 2016 in Bethesda, MD. Uniquely focused on in vitro testing, the workshop is the only known event of its kind.Attendees, including toxicologists from industry, the FDA Center for Tobacco Products, the National Center for Toxicological Research and the US Environmental Protection Agency, heard presentations, attended poster sessions and breakout groups to gain a deeper understanding of the issues involving exposure and dosimetry of aerosols to cells and tissues in culture. Topics included the current status of in vitro to in vivo correlations, whole tobacco smoke and E-cigarette aerosol/vapor constituents, in vitro exposure systems, dosimetry approaches, the exposure microenvironment, and promising technologies that may advance science in these areas.To date, much of the research and testing in respiratory and inhalation toxicology have focused on the use of animal models. With the development of new technologies, such as reconstructed human airway tissues, researchers are turning their attention to in vitro assessments. 

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