A Screening Method by Gas Chromatography-Mass Spectrometry for the Quantification of 24 Aerosol Constituents from Heat-Not-Burn Tobacco Products

Authored by  I Hofer, L Gautier, E. Sauteur, M. Dobler, A. Python, C O’Reilly, D Gisi, E Tinguely, L. Wehren, E. Fidalgo

Published in Beiträge zur Tabakforschung International/ Contributions to Tobacco Research    


A screening method allowing the quantification of 24aerosol constituents using gas chromatography-mass spec-trometry has been developed to assess the aerosol chemis-try of heat-not-burn tobacco products. The aim of this method was to quantify phenol, o-cresol,m-cresol, p-cresol, catechol, resorcinol, hydroquinone,1,3-butadiene, isoprene, benzene, acrylonitrile, toluene,pyridine, styrene, 1,2-propylene glycol, menthol, 2-furan-methanol, acrylamide, naphthalene, nicotine, acetamide, quinoline, triacetin, and glycerine in the aerosol emitted by heated tobacco products. The aerosol was generated by annelectrically heated tobacco system (PMI’s Heated Tobacco System (THS 2.4)) with one single aerosol collection method, using the Health Canada smoking regimen and analyzed with two analytical methods. The method was validated according to the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use and the Association of Official Analytical Chemists guidelines. A regression model based on a linear relationship between concentration and response ratio with a 1/x weighting factor was selected for phenol, o-cresol, m-cresol, p-cresol,1,3-butadiene, isoprene, benzene, acrylonitrile, toluene, pyridine, styrene, 2-furanmethanol, acrylamide, naphtha-lene and acetamide. A quadratic regression model with a1/x weighting factor was chosen for catechol, resorcinol, hydroquinone, 1,2-propylene glycol, menthol, nicotine, quinoline, triacetin and glycerine. Coefficients of variation for repeatability were determined between 7.9% and 17.8%and for intermediate precision between 8.1% and 19.9%