Advances in Nicotiana Genetic and “Omics” Resources
Published in Genomics of Plant Genetic Resources Volume 1. Managing, sequencing and mining genetic resources pp 511-532
The importance of the genus Nicotiana is both economic as it contains species which are major cash crops, and scientific, as many of the species' genomes are highly complex due to their evolutionary history. Investigating these species has been accelerated by advances in "Omics" techniques; these high-throughput methods, nucleic acid sequencing in particular, have led to an explosion in the volume of data available.
Here we provide an overview of how these data are organized and stored as public genome resources, as well as how Nicotiana researchers can mine or otherwise use these data to answer scientific questions, such as by constructing microarrays from expressed sequence tag (EST) and genomic data. We further examine past sequencing efforts, such as the Tobacco Genome Initiative (TGI), and the objectives and progress of current projects in the field, in particular for N. tabacum, N. sylvestris, N. tomentosiformis, and N. benthamiana, which are part of the SOL 100 target list. We look at how these data can be leveraged in the future, as for example by using genomic sequences for proteogenomics, or creating species-specific metabolic pathway repositories generated from genome annotation data. Nicotiana genetic and "Omics" resources can be used to improve breeding strategies to obtain desirable traits such as disease and stress resistance, yield and quality.
Published OnJanuary 1, 2013