Analysis of TSNA in THS aerosol

Authored by  PMI

The aerosol samples are generated with a linear smoking machine and collected on a Cambridge filter pad. Target compounds are then extracted with a 100 mM ammonium acetate solution containing the internal standards.
The extracts are analyzed by High Performance Liquid Chromatography (HPLC) using a tandem mass spectrometry with an Heat Electrospray Ionization (HESI) interface in positive mode. The HPLC system is equipped with a column Supelco Discovery HC-C18 column, 50 x 2.1 mm ID x 3.0 μm.
Results are expressed as ng/item for Platform 1.