Development of two different methods to characterize the chemical composition of cigarette mainstream smoke
Presented at Frontiers in Aerosol Dosimetry Research
In order to characterize the main stream smoke while preserving its chemical compositions, two different approaches were developed. The first method is based on the trapping of the whole smoke at -183°C followed by the quantification of several smoke components using selected analytical techniques. The trapping is performed with a cryogenic instrument enabling a precise control of the temperature down to values as low as -190°C. After the trapping, the condensed smoke is diluted with a solvent and selected smoke components can be quantified using gas chromatography-mass spectrometry and liquid chromatography. The results obtained when trapping the main stream smoke of the 2R4F reference cigarette at -183°C were compared with those obtained by the standard methods. A good agreement was observed between the two approaches for 17 analytes, including some aldehydes, olefins, aromatic compounds and poly-aromatic compounds. The second method was designed to analyze the chemical composition of a single puff using gas chromatography-mass spectrometry. The smoke is sampled with a gas syringe, while the puff is being drawn. In order to evaluate the maximum number of puffs during one smoking run, an ultra-fast gas chromatograph-time of flight-mass spectrometer was used to analyze the smoke composition. The ultra-fast module enabled to analyze VOCs from isoprene to diterpenes in 1 minute. The evolution of the puff composition in the course of a smoking run was studied for the 2R4F reference cigarette, on components of both the gas and the particulate phases.