Identification of gene expression signature for cigarette smoke exposure response-from man to mouse

Authored by  F Martin, M Talikka, J Hoeng, M Peitsch

Published in Human & Experimental Toxicology     


Gene expression profiling data can be used in toxicology to assess both the level and impact of toxicantexposure, aligned with a vision of 21st century toxicology. Here, we present a whole blood-derived gene signaturethat can distinguish current smokers from either nonsmokers or former smokers with high specificityand sensitivity. Such a signature that can be measured in a surrogate tissue (whole blood) may help in monitoringsmoking exposure as well as discontinuation of exposure when the primarily impacted tissue (e.g., lung)is not readily accessible. The signature consisted of LRRN3, SASH1, PALLD, RGL1, TNFRSF17, CDKN1C, IGJ,RRM2, ID3, SERPING1, and FUCA1. Several members of this signature have been previously described in thecontext of smoking. The signature translated well across species and could distinguish mice that were exposedto cigarette smoke from ones exposed to air only or had been withdrawn from cigarette smoke exposure.Finally, the small signature of only 11 genes could be converted into a polymerase chain reaction-based assaythat could serve as a marker to monitor compliance with a smoking abstinence protocol.