iTRAQ approach to investigate the impact of cigarette smoke, smoking cessation and switching to a prototypic modified risk tobacco product on the lung proteome of C57BL/6 mice

Authored by  E Veljkovic, J Hoeng, M Peitsch, NV Ivanov, F Martin


Reliable system-wide measurements are key to a meaningful systems-toxicology approach by which impact of toxicants in cells are evaluated (Hoeng et al., 2012). While still being challenging to setup, liquid-chromatography mass-spectrometry (LC-MS) based quantitative proteomics may lead to a further understanding of systems response and complement the widely used transcriptomics data. In the context of a life-long animal study aiming at characterizing the impact of cigarette smoke in mouse lung and the effect of cessation and switching to a prototypic Modified Risk Tobacco Product (pMRTP), we established an isobaric tags for absolute and relative quantification (iTRAQ®)-based pipeline aiming at capturing a wide variety of proteins. The arms of  this animal study involving C57/Bl6 mice were exposure to: (i) fresh-air only, (ii) conventional cigarette smoke (CS) from 3R4F cigarettes (from University of Kentucky),  (iii) CS and switch to a pMRTP,  (iv) CS and switch to fresh air (cessation) and (v) pMRTP only.