Watch our video presentation explaining our computational approach for using molecular data to determine the comparative biological impact of cigarette smoke and RRP aerosol.
There are four steps to the computational component of our systems toxicology approach to Reduced-Risk Product (RRP) assessment.
Firstly, we generate Systems Response Profiles, which are a measure of the degree to which individual molecular entities are changed as a consequence of exposure to specific compounds.
Secondly, we build models of the biological processes relevant to smoking-related diseases, providing a layer of mechanistic understanding that allows us to go beyond the examination of single genes.
Thirdly, we calculate Network Perturbation Amplitudes (NPAs), which describe the magnitude of biological network alterations brought about by exposing tissue to a given stimulus.
Finally, NPAs are aggregated into the Biological Impact Factor representing the overall alteration of the biological system being studied as a result of exposure to RRP aerosols, exposure to cigarette smoke or smoking cessation.