Presentations

      A GC-HR-TOF-MS Method To Semi-Quantify Constituents In Aerosols And Aerosol Fractions

      Dossin, E.; Monge, A.; Martin, E.; Pospisil, P.; Knorr, A.; Bentley, M. C.; Guy, P. A.
      Conference date
      Jun 19, 2013
      Conference name
      ACD/LABS Symposium On Laboratory Intelligence (EUM) 
      Link to more information
      Link to more information
      Topic
      Summary

      In order to demonstrate that Modified Risk Tobacco Products (MRTPs) have the potential to deliver risk reduction when used in place of conventional cigarettes, it is crucial to perform a comprehensive chemical characterization of the aerosols produced by these products. This knowledge will enable a comparison of the different physicochemical properties of aerosol composition, both to optimize our MRTPs and to interpret gene expression results from in vitro and in vivo studies (why and how the different network or pathway perturbations are affected). Orthogonal approaches are used for aerosol characterization and here we report on a fingerprinting method to semi-quantify several hundred aerosol constituents using gas chromatography coupled to high resolution time-of-flight mass spectrometry (GC-HR-TOF-MS) employing isotopic dilution has been developed. This method combines the use of an in-house built chemical master database ‘Unique Compound Structure Database (UCSD)’ with a Quantitative Structure Property Relationship (QSPR) model to build the capability for retention time prediction from the analysis of reference standard compounds for new compound identification. A total of approximately 500 reference standard compounds (including isotopically labeled internal standards) were analyzed by GC-HR-TOF-MS using a DB5-MS GC column while the HR-TOF-MS instrument was operating under electron impact ionization. The electron impact mass spectra of reference compounds were registered in our SpecDb master database (UCSD). Using the prediction model, targeted almost thousand aerosol constituents were identified and categorized into several compound classes according to their physicochemical properties using accelrys pipeline pilot software and used for product comparisons. Semi-quantification was achieved using specific isotopically labeled internal standards representative of each compound class, with the potential of quantifying deliveries of individual aerosol constituents in the region of ng per tobacco product. 

      PMIScience.com is operated by Philip Morris International for the purpose of publishing and disseminating scientific information about Philip Morris International’s efforts in support of its smoke-free product portfolio. This site is a global site for use by scientists, the public health and regulatory communities, and other stakeholders with an interest in tobacco policy. The purpose of this site is not advertising or marketing, nor is it directed at any specific market. It is not intended for use by consumers. New tobacco products sold in the United States are subject to FDA regulation; therefore the content of this site is not intended to make, and nor should it be construed as making, any product related claims in the United States without proper FDA authorization. ​
      ​
      Reduced Risk Products ("RRPs”) is the term we use to refer to products that present, are likely to present, or have the potential to present less risk of harm to smokers who switch to these products versus continuing smoking. PMI has a range of RRPs in various stages of development, scientific assessment and commercialization. All of our RRPs are smoke-free products that deliver nicotine with far lower quantities of harmful and potentially harmful constituents than found in cigarette smoke.