Methods and Protocols

      Analysis of 12 polycyclic aromatic hydrocarbons in THS aerosol


      Published on
      Mar 14, 2017

      The aerosol samples are generated with a linear smoking machine and collected on a Cambridge filter pad. Polycyclic aromatic hydrocarbons (PAHs) are then extracted with hexane after addition of internal standards. The aerosol extract undergoes a two steps solid-phase extraction (SPE) clean-up procedure in order to remove some matrix co-extracted organic compounds that reduce the performance of the chromatographic system and affect the accuracy of PAHs determination. The resulting extracts are analyzed by Gas Chromatography with Electron Impact Mass Spectrometry (GCEI-MS). The GC system is equipped with a column DB-17MS column, 30 m x 0.25 mm ID x 0.15 μm (film thickness). Results are expressed as ng/item for the Tobacco Heating System (THS), referred to as Platform 1 (P1) in the method.