Methods and Protocols

      Analysis of TSNA in THS aerosol

      PMI

      Published on
      Mar 14, 2017
      Topic
      Summary

      The aerosol samples are generated with a linear smoking machine and collected on a Cambridge filter pad. Target compounds are then extracted with a 100 mM ammonium acetate solution containing the internal standards. The extracts are analyzed by High Performance Liquid Chromatography (HPLC) using a tandem mass spectrometry with an Heat Electrospray Ionization (HESI) interface in positive mode. The HPLC system is equipped with a column Supelco Discovery HC-C18 column, 50 x 2.1 mm ID x 3.0 μm. Results are expressed as ng/item for the Tobacco Heating System (THS), referred to as Platform 1 (P1) in the method.