Peer-Reviewed Publications

    Process development for efficient pectin extraction from tobacco residues and its characterisation

    Bedzo, O. K. K.; Gottumukkala, L. D.; Lo Sasso, G.; Kaminski, K.; Schlage, W.; Goffman, F.; Ivanov, N.; Hoeng, J.; Hayes, D. J.  

    Published
    Aug 19, 2023
    DOI
    10.1007/s13399-023-04750-4
    Link to publication
    Topic
    Summary

    This study presents a method for effective pectin extraction from the laminae of three tobacco varieties as a means of biomass valorisation. Two pre-treatment methods (cold ethanol vs. accelerated solvent extraction [ASE] with ethanol) were compared for their capacities to produce a high pectin yield. Enzymatic extraction of pectin was also tested as a green extraction procedure and compared to the acid extraction approach. The optimisation experiments revealed that cold ethanol extraction followed by acid hydrolysis is the most convenient method for pectin extraction; the optimal set of conditions for hydrolysis were identified as 90°C, pH 1.5, and 4 h of extraction. Applying these optimised conditions to the three Nicotiana rustica tobacco varieties yielded pectin recoveries of 66.2%, 57.8%, and 56.7% from the NRT63, Bakoum Miena, and NRT61 samples, respectively. Tobacco pectins were found to have a medium molecular weight and low methoxy content. These results highlight the potential of tobacco residues as feedstock for to produce pectin with dietary applications.

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